Size (kb): 8.3999996185302730

Vector: YEp368R (plasmid)

Promoters: Promoter none

Construction: YEp363A, YEp358R (ATCC 37739)

Marker(s):LEU2,ampR

Construct size (kb): 8.399999618530273

Features: insert detection: lacZ
marker(s): ampR, LEU2
promoter: none
replicon: pMB1, 2 micron

YE-type (episomal) shuttle vector

promoter-cloning vector

shuttle vector

Cloning into the HindIII, SphI, PstI, SalI, or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region.

The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-2; SphI-3; PstI-3; SalI-2; XbaI-2; BamHI-2; SmaI-3; KpnI-3; SacI-3; EcoRI-2. The EcoRI, KpnI, and SacI sites are not unique.

Restriction digests of the clone give the following sizes (kb): HindIII--8.4; BamHI--8.4; PstI--8.4.

One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37740 - 37742) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.

The sequence and reading frame of the multiple cloning sequence is: 5'AA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT CCC AGC TTC GAT CCC3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.

Temperature: 37.0°C