Size (kb): 5.616

Vector: pRS424 (phagemid)

Promoters: Promoter T7 (phi10)

Construction: pJS92, pRS304, pBluescript II SK+

Marker(s):TRP1,ampR

Construct size (kb): 5.616000175476074

Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: T3
promoter: T7 (phi10)
promoter: lac
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: KpnI...SacI

YE-type (episomal) shuttle vector

shuttle vector

vector containing primer sites useful for sequencing

vector permitting RNA synthesis in vitro

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; PvuII--5.2, 0.5; XbaI--3.3, 1.4, 1.0.

Contains the REP3 and FRT sequences necessary for high copy propagation in yeast.

In S. cerevisiae, the number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 1.5 + or - 1.7% of progeny per doubling.

YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing.

The order of the major features in this plasmid is: ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori(2 micron) - TRP1.

Temperature: 37.0°C

component of:ATCC 77108

component of:ATCC 87532

component of:ATCC 87535